human normal colon smooth muscle cells  (Procell Inc)

Journal: Oncogene

Article Title: Genomic evolution in Barrett’s adenocarcinoma cells: critical roles of elevated hsRAD51, homologous recombination and Alu sequences in the genome

doi: 10.1038/onc.2011.83

Figure Lengend Snippet: Recombinase (hsRAD51) is overexpressed in Barrett’s esophageal adenocarcinoma (BAC) cell lines and tissue specimens. ( a ) RAD51 protein expression was analyzed by western blotting in normal human fibroblasts (FIBS), normal primary HEEC, BEAC cell lines (FLO-1, OE33), FLO-1 treated with recombinogen nickel chloride (0.6 mg/ml) for 3 h, normal human colonic smooth muscle cells (HCSMCs) and colonic adenocarcinoma cell lines (SW480, SW620, HCT116). (b) Bar graph showing fold elevation in RAD51 in the adenocarcinoma cell lines of panel ( a ), relative to control cells. (c ) RAD51 immunostaining within BEAC, vs normal tissue. Sections of paraffin-embedded BAC tissues were deparaffinized and treated with anti-RAD51 mouse monoclonal antibody. The samples were then treated with Alexa-Fluor 488-labeled goat anti-mouse secondary antibody and viewed under a fluorescence microscope. The pictures show fluorescence merged with transmitted-light images. (d ) Table showing relative expression of RAD51 in all human tissue specimens examined, including those of panel ( c ). BE, Barrett’s esophagus; BAC Barrett’s adenocarcinoma. A full colour version of this figure is available at the Oncogene journal online.

Article Snippet: Colon cancer cell lines SW480, SW620, HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA) and normal human colonic smooth muscle cells from ScienCell Research Laboratories.

Techniques: Expressing, Western Blot, Control, Immunostaining, Labeling, Fluorescence, Microscopy